Active Recombinant Human KIRREL Protein, His-tagged

• Cat.No.: KIRREL-719H
• Product Overview: Recombinant human KIRREL (NP_060710.3) (Met1-Leu493) was expressed with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-493 a.a.
• Predicted N Terminal: Gln 17
• Form: Lyophilized from sterile PBS, pH 7.4, 5% ~ 8% trehalose and mannitol.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of MS1 mouse pancreatic islet endothelial cells. When cells are added to KIRREL coated plates (15 μg/mL, 100 μL/well), >20% will adhere specifically after 90 minutes at 37 °C.
• Molecular Mass: The recombinant human KIRREL consists 488 amino acids and predicts a molecular mass of 53.5 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: KIRREL kin of IRRE like (Drosophila) [ Homo sapiens ]
• Official Symbol: KIRREL
• Synonyms: KIRREL; kin of IRRE like (Drosophila); kin of IRRE-like protein 1; NEPH1; nephrin like protein 1; nephrin related; nephrin-like protein 1; kin of irregular chiasm-like protein 1; FLJ10845; MGC129542; MGC129543
• Gene ID: 55243
• mRNA Refseq: NM_018240
• Protein Refseq: NP_060710
• MIM: 607428
• UniProt ID: Q96J84
• Chromosome Location: 1q21-q25
• Pathway: Cell-Cell communication, organism-specific biosystem; Nephrin interactions, organism-specific biosystem; Nephrin/Neph1 signaling in the kidney podocyte, organism-specific biosystem