Active Recombinant Human KIRREL, His-tagged

• Cat.No.: KIRREL-5225H
• Product Overview: Recombinant Human KIRREL1 /NEPH1 Protein (rhKIRREL1) Gln 17 - Ala 499 (Accession # AAI09193.1) was produced in human 293 cells (HEK293)

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 17-499 a.a.
• Description: Kin of IRRE-like protein 1 (KIRREL1) also known as NEPH1, is type I transmembrane glycoproteina that belongs to the nephrin-like protein family, which includes NEPH2 and NEPH3 (KIRREL2). The cytoplasmic domains of these proteins interact with the C terminus of podocin (NPHS2). KIRREL1 is expressed in filtration slits of kidney podocytes, cells involved in ensuring size- and charge-selective ultrafiltration of blood. KIRREL1 forms cis hetero­oligomers with nephrin, which brings together signaling molecules that direct actin polymerization. This interaction is essential for barrier function in the slit diaphragm, and mice deleted for KIRREL1 die perinatally due to proteinuria and failure to thrive. KIRREL1 is crucial for the integrity of the slit diaphragm, as KIRREL gene knockout mice results in effacement of glomerular podocytes, heavy proteinuria, and early postnatal death.
• Predicted N Terminal: Gln 17
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of MS-1 mouse pancreatic islet endothelial cells.When 50000 cells/well are added to rhKIRREL1 coated plates (30 μg/mL, 100 μg/well), approximately 50%-75% will adhere after 90 minutes at 37℃.
• Molecular Mass: 53.5 kDa. DTT-reduced Protein migrates as 65-70 kDa due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg of the rhKIRREL1 by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC-8oC); After reconstitution under sterile conditions for 1 month (4oC-8oC) or 3 months (-20oC to -70oC).

Gene Information

• Gene Name: KIRREL kin of IRRE like (Drosophila) [ Homo sapiens ]
• Official Symbol: KIRREL
• Synonyms: KIRREL; kin of IRRE like (Drosophila); kin of IRRE-like protein 1; NEPH1; nephrin like protein 1; nephrin related; nephrin-like protein 1; kin of irregular chiasm-like protein 1; FLJ10845; MGC129542; MGC129543
• Gene ID: 55243
• mRNA Refseq: NM_018240
• Protein Refseq: NP_060710
• MIM: 607428
• UniProt ID: Q96J84
• Chromosome Location: 1q21-q25
• Pathway: Cell-Cell communication, organism-specific biosystem; Nephrin interactions, organism-specific biosystem; Nephrin/Neph1 signaling in the kidney podocyte, organism-specific biosystem