Active Recombinant Human Killer Cell Immunoglobulin-Like Receptor, Two Domains, Long Cytoplasmic Tail, 5A, Fc-tagged

• Cat.No.: KIR2DL5A-5668H
• Product Overview: RecombinantHuman KIR2DL5A protein was expressed in Murine myeloma cell line with the Fcpart of Human IgG1 Pro100-Lys330 (C-terminus) and Human KIR2DL5A His22- His240 (N-terminus).

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: Fc
• Description: KIR2DL5Ais a 60 kDa type I transmembrane glycoprotein that belongs to the killer cellIg-like receptor (KIR) family. KIRs are expressed on CD56 dim NK cells and Tcell subsets where they regulate effector functions in the innate immunesystem. KIRs are named for the number of Ig-like domains (2D or 3D) in theextracellular domain (ECD), and whether they have long or short (L, S)cytoplasmic tails. Like other inhibiting KIRs, KIR2DL5A has two ITIM domainswithin its long tail however, one of these is atypical and may cause KIR2DL5to be less inhibitory than most inhibiting KIRs.
• Form: Lyophilizedfrom a 0.2 μm filtered solution in PBS.
• N-terminalSequence: His22
• MolecularWeight: 50.1 kDa (monomer)
• Activity: Measuredby its ability to bind HLA on MDA-MB-231 human breast adenocarcinoma cells. TheED50 for this effect is typically 0.9-3.6 μg/mL.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 66 kDa,reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 300 μg/mLin PBS
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to-70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: KIR2DL5A killer cellimmunoglobulin-like receptor, two domains, long cytoplasmic tail, 5A [ Homosapiens ]
• Official Symbol: KIR2DL5A
• Synonyms: KIR2DL5A; killercell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 5A; CD158F; KIR2DL5; KIR2DL5B; KIR2DL5.1;KIR2DL5.3; killer cell immunoglobulin-like receptor 2DL5A; killer-cellIg-like receptor; killer cell immunoglobulin-like receptor KIR2DL5A; killercell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 5; CD158f1; CD158F1
• Gene ID: 57292
• mRNA Refseq: NM_020535
• Protein Refseq: NP_065396
• MIM: 605305
• UniProt ID: Q8N109
• Chromosome Location: 19q13.4
• Pathway: Antigen processingand presentation; Graft-versus-host disease; Natural killer cell mediatedcytotoxicity
• Function: receptor activity