Active Recombinant Human JAG1 protein, His-tagged

• Cat.No.: JAG1-3226H
• Product Overview: Recombinant Human JAG1 protein(Gln 34 - Ser 1046), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 34-1046 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Human Jagged 1, His Tag immobilized on CM5 Chip can bind Human NOTCH1, Fc Tag with an affinity constant of 5.13 μM as determined in a SPR assay (Biacore T200).
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 112.3 kDa. The protein migrates as 130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Gln34-Ser1046
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: JAG1 jagged 1 [ Homo sapiens ]
• Official Symbol: JAG1
• Synonyms: JAG1; jagged 1; AGS, Alagille syndrome , JAGL1; protein jagged-1; AHD; AWS; CD339; HJ1; AGS; JAGL1; MGC104644
• Gene ID: 182
• mRNA Refseq: NM_000214
• Protein Refseq: NP_000205
• UniProt ID: P78504