Active Recombinant Human ITGAV Protein, C-6×His-tagged

• Cat.No.: ITGAV-26H
• Product Overview: Recombinant Human Integrin alpha V (Phe31-Val992, Acidic Tail) with a 6×His tag at C-terminus and Human Integrin beta 1 (Gln21-Asp728, Basic Tail) was expressed in Chinese Hamster Ovary cell line.

Specification

• Description: The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha V subunit. This subunit associates with beta 1, beta 3, beta 5, beta 6 and beta 8 subunits. The heterodimer consisting of alpha V and beta 3 subunits is also known as the vitronectin receptor. This integrin may regulate angiogenesis and cancer progression. Alternative splicing results in multiple transcript variants. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes.
• Source: CHO
• Species: Human
• Tag: His
• Form: Noncovalently-linked heterodimer
• Bio-activity: Measured by its binding ability in a functional ELISA. When Recombinant Human Fibronectin is immobilized at 2 μg/mL, 100 μL/well, Recombinant Human Integrin alpha V beta 1 binds with an ED50 of 0.08-0.48 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 115 kDa ( alpha V subunit) & 86 kDa ( beta 1 subunit)
  SDS-PAGE: 120-170 kDa, reducing conditions
• Protein length: Integrin alpha V: Phe31-Val992
  Integrin beta 1: Gln21-Asp728
• N-terminal Sequence Analysis: Phe31 ( alpha V subunit) & Gln21 ( beta 1 subunit, inferred from enzymatic pyroglutamate treatment revealing Thr22)
• Endotoxin: <1.0 EU/μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Reconstitution: Reconstitute at 500 μg/mL in water.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: ITGAV integrin subunit alpha V [ Homo sapiens (human) ]
• Official Symbol: ITGAV
• Synonyms: ITGAV; integrin subunit alpha V; integrin alpha-V; antigen identified by monoclonal antibody L230; integrin alphaVbeta3; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); vitronectin receptor subunit alpha
• Gene ID: 3685
• mRNA Refseq: NM_002210
• Protein Refseq: NP_002201
• MIM: 193210
• UniProt ID: P06756