Active Recombinant Human ITGAV & ITGB8 Heterodimer Protein, His-tagged

• Cat.No.: ITGAV&ITGB8-366H
• Product Overview: Recombinant Human ITGAV & ITGB8 Heterodimer Protein, His-tagged is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 992 (ITGAV) & Glu 43 - Arg 684 (ITGB8) (Accession # NP_002201 (ITGAV) & NP_002205 (ITGB8)). Predicted N-terminus: Phe 31 (ITGAV) & Glu 43 (ITGB8). Human ITGAV & ITGB8 Heterodimer Protein, produced by co-expression of ITGAV and ITGB8, has a calculated MW of 113.0 kDa (ITGAV) and 76.5 kDa (ITGB8). Subunit ITGAV is fused with polyhistidine tag at the C-terminus and followed by a acidic tail and subunit ITGB8 contains no tag but a basic tail at the C-terminus. The reducing (R) protein migrates as 120-130 kDa and 140-150 kDa (ITGAV) and 90-95 kDa (ITGB8) respectively due to glycosylation.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• ProteinLength: Phe 31 - Val 992 (ITGAV) & Glu 43 - Arg 684 (ITGB8)
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM NaCl, 2 mM MgCl2, pH8.0. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Recombinant Human ITGAV & ITGB8 Heterodimer Protein, His-tagged at 5 μg/mL (100 μL/well) can bind Biotinylated Human TGF-Beta 1, His Tag, Avi Tag with a linear range of 0.8-13 ng/mL (QC tested).
  Immobilized Human Fibronectin at 10 μg/mL (100 μL/well) can bind Recombinant Human ITGAV & ITGB8 Heterodimer Protein, His-tagged with a linear range of 0.313-10 μg/mL (Routinely tested).
  Immobilized Human Vitronectin at 5 μg/mL (100 μL/well) can bind Recombinant Human ITGAV & ITGB8 Heterodimer Protein, His-tagged with a linear range of 0.313-10 μg/mL (Routinely tested).
• Molecular Mass: 113.0 kDa (ITGAV) & 76.5 kDa (ITGB8)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: ITGAV integrin, alpha V [ Homo sapiens ] & ITGB8 integrin, beta 8 [ Homo sapiens ]
• Official Symbol: ITGAV & ITGB8
• Synonyms: ITGAV; integrin, alpha V; CD51; MSK8; VNRA; VTNR; DKFZp686A08142; & ITGB8; integrin, beta 8; integrin beta-8
• Gene ID: 3685 & 3696
• mRNA Refseq: NM_001144999 & NM_002214
• Protein Refseq: NP_001138471 & NP_002205
• MIM: 193210 & 604160
• UniProt ID: P06756 & P26012