Active Recombinant Human Interleukin 17F

• Cat.No.: IL17F-114H
• Product Overview: Recombinant Human Interleukin 17F encoding the human IL-17F protein sequence (containing the signalpeptide sequence, and the mature IL-17F sequence) was expressed in modifiedhuman 293 cells.

Specification

• Cat. No.: IL17F-114H
• Description: Interleukin-17 (IL-17) is a 155 amino acid protein that is a disulfide linked, homodimeric, secreted glycoprotein with one N-linked glycosylation site. IL-17 is also referred to as IL-17A. Other members include IL-17B, IL-17C, IL-17D, IL-17E and IL-17F. IL-17 binds to the cell surfacer receptor IL-17R. IL-17 is secreted by a subset of T cells called T-helper 17 (Th-17) cells. IL-23 is thought to mediate the production of IL-17 by Th-17 cells.
• Source: Human 293 cells.
• Amino Acid Sequence: RKIPKVGHTFFQKPESCPPVPGGSMKLDIGIINENQRVSMSRNIESRSTSPWNYTVTWDPNRYPSEVVQAQCRNLGCINAQGKEDISMNSVPIQQETLVVRRKHQGCSVSFQLEKVLVTVGCTCVTPVIHHVQ.
• Molecular Mass: Under reducing conditions Apollo IL-17F hcx migrates as a broad band between 17 and 21 kDa on SDS-PAGE due to post-translational modifications, in particular glycosylation. This compares with unmodified IL-17F polypeptide that has a predicted monomeric molecular mass of 14.9 kDa.
• pI: Apollo IL-17F hcx separates into a number of glycoforms with a pI between 6.2 and 9.5 on 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified IL-17F that has a predicted pI of 9.04.
• % Carbohydrate: Apollo purified IL-17F hcx consists of 20-35% carbohydrate by weight.
• Glycosylation: Apollo IL-17F hcx contains N-linked oligosaccharides.
• Purity: >95%, as determined by SDS-PAGE, visualised by Coomassie Brilliant Blue.
• Formulation: When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
• Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
• Storage: Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C and longer-term storage of aliquots at -18 to -20°C is recommended. Repeated freeze thawing is not recommended.
• Activity: The activity of IL-17F is measured by its ability to induce IL-6 production in NHDF cells. The ED₅₀is typically between 0.04 and 0.1 ng/ml.
• Tag: Non

Gene Information

• Gene Name: IL17F interleukin 17F [ Homo sapiens ]
• Synonyms: IL17F; interleukin 17F; ML1; ML-1; IL-17F; cytokine ML-1;Interleukin-24;leukin-17F
• Gene ID: 112744
• mRNA Refseq: NM_052872
• Protein Refseq: NP_443104
• UniProt ID: Q96PD4
• Chromosome Location: 6p12
• MIM: 606496
• Function: cytokine activity; cytokine binding; protein homodimerization activity