Active Recombinant Human IL17F protein, His-tagged

• Cat.No.: IL17F-307H
• Product Overview: Recombinant Human IL17F protein(Arg 31 - Gln 163 ), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human IL-17F (H161R), His Tag at 10 μg/mL (100 μL/well) can bind Biotinylated Human IL-17 RA, Fc,Avitag with a linear range of 0.02-0.313 μg/mL.Immobilized Human IL-17F (H161R), His Tag at 10 μg/mL (100 μL/well) can bind Human IL-17 RA, Fc Tag with a linear range of 0.078-1.25 μg/mL.Immobilized Human IL-17F (H161R), His Tag at 2 μg/mL (100 μL/well) can bind Recombinant Human IL-17RC Isoform 1 Fc Chimera with a linear range of 0.2-16 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the N-terminus.The protein has a calculated MW of 16.8 kDa. The protein migrates as 23 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Arg 31 - Gln 163
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL17F interleukin 17F [ Homo sapiens ]
• Official Symbol: IL17F
• Synonyms: IL17F; interleukin 17F; interleukin-17F; IL 17F; ML 1; ML1; IL-24; cytokine ML-1; mutant IL-17F; interleukin-24; ML-1; CANDF6; IL-17F
• Gene ID: 112744
• mRNA Refseq: NM_052872
• Protein Refseq: NP_443104
• MIM: 606496
• UniProt ID: Q96PD4