Active Recombinant Human INHBA

• Cat.No.: INHBA-141H
• Product Overview: Active form Activin-A Human Recombinant produced in e.coli is a homodimeric, non-glycosylated, polypeptide chain containing 2 x 117 amino acids and having a molecular weight of 26.2kDa. The Active form Activin-A is purified by standard chromatographic techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: The inhibin beta A subunit joins the alpha subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumor-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. Because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone. Furthermore, the beta A subunit forms a homodimer, activin A, and also joins with a beta B subunit to form a heterodimer, activin AB, both of which stimulate FSH secretion. Finally, it has been shown that the beta A subunit mRNA is identical to the erythroid differentiation factor subunit mRNA and that only one gene for this mRNA exists in the human genome.
• Form: Lyophilized freeze dried powder. Human Activin-A was lyophilized from a concentrated 1mg/ml protein solution containing 0.02% TFA.
• Bio-activity: Biological activity is assessed by the ability to induce cytoxicity of MPC-11 cells and was found to be 1-1.5 ng/ml corresponding to a specific activity of 666,667-1,000,000 Units/mg.
• Molecular Mass: 26.2kDa.
• AA Sequence: MGLECDGKVN ICCKKQFFVS FKDIGWNDWI IAPSGYHANY CEGECPSHIA GTSGSSLSFH STVINHYRMR GHSPFANLKS CCVPTKLRPM SMLYYDDGQN IIKKDIQNMI VEECGCS.
• Purity: Greater than 95% as obsereved by SDS-PAGE.
• Stability: Lyophilized Activin-A although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Activin-A should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
• Reconstitution: Human INHBA protein should be reconstituted in distilled pyrogen free water to a concentration of 100ug /ml which can then be further diluted to other aqueous solutions.

Gene Information

• Gene Name: INHBA inhibin, beta A [ Homo sapiens (human) ]
• Official Symbol: INHBA
• Synonyms: INHBA; inhibin, beta A; EDF; FRP; Inhibin, beta-1; OTTHUMP00000207260; FSH-releasing protein; erythroid differentiation factor; follicle-stimulating hormone-releasing protein; inhibin, beta A (activin A, activin AB alpha polypeptide); Activin beta-A chain; Erythroid differentiation protein; FSH-releasing protein
• Gene ID: 3624
• mRNA Refseq: NM_002192
• Protein Refseq: NP_002183
• MIM: 147290
• UniProt ID: P08476
• Chromosome Location: 7p15-p13
• Pathway: ALK1 signaling events; Cardiac Progenitor Differentiation; Metabolism of proteins
• Function: cytokine activity; growth factor activity; identical protein binding