Active Recombinant Human IL34 Protein (Asn21-Pro242), C-His tagged, Animal-free, Carrier-free

• Cat.No.: IL34-31H
• Product Overview: Recombinant Human IL34 Protein (Asn21-Pro242) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Interleukin-34 (IL-34) is a 30 kDa cytokine with 223 amino acid residues. IL-34 is a ligand of CSF-1R and secreted from CSF-1R expressing cells like myeloid cells, epithelial cells, endothelial cells, fibroblasts, and neurons. IL-34 mediates cell survival, proliferation, cytokine/chemokine expression, and migration via activating several signaling pathways. Moreover, it also plays a critical role in developing and maintaining the mononuclear phagocytic system.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce human peripheral blood monocytes (PBMCs) proliferation. The ED50 for this effect is < 30 pg/mL.
• Molecular Mass: The protein has a calculated MW of 26.17 kDa.
  The protein migrates as 30 kDa under reducing condition (SDS-PAGE analysis).
• Protein length: Asn21-Pro242
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: IL34 interleukin 34 [ Homo sapiens (human) ]
• Official Symbol: IL34
• Synonyms: IL34; interleukin 34; C16orf77, chromosome 16 open reading frame 77; interleukin-34; IL 34; MGC34647; IL-34; C16orf77
• Gene ID: 146433
• mRNA Refseq: NM_001172771
• Protein Refseq: NP_001166242
• MIM: 612081
• UniProt ID: Q6ZMJ4