Active Recombinant Human IL31 Protein (Ser24-Thr164), C-His tagged, Animal-free, Carrier-free

• Cat.No.: IL31-187H
• Product Overview: Recombinant Human IL31 Protein (Ser24-Thr164) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Interleukin 31 (IL-31) is an 18 kDa cytokine with 142 amino acid residues. IL-31 is mainly secreted from activated CD4+ T cells and binds to a receptor called IL-31RA. Upon binding to 31RA, IL-31 activates several signal pathways like MAPK, PI3K/AKT, and Jak/STAT pathways. It is critical in regulating many biological functions, such as cell proliferation, hematopoiesis, induction of cytokines, inflammation, and immune response.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized
• Bio-activity: Measured by its ability to induce A549 cells proliferation. The ED50 for this effect is < 40 ng/mL.
• Molecular Mass: The protein has a calculated MW of 16.78 kDa.
  The protein migrates as 18 kDa under reducing condition (SDS-PAGE analysis).
• Protein length: Ser24-Thr164
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: IL31 interleukin 31 [ Homo sapiens (human) ]
• Official Symbol: IL31
• Synonyms: IL31; interleukin 31; interleukin-31; IL 31; IL-31
• Gene ID: 386653
• mRNA Refseq: NM_001014336
• Protein Refseq: NP_001014358
• MIM: 609509
• UniProt ID: Q6EBC2