Active Recombinant Human IL27 protein, His-tagged

• Cat.No.: IL27-881H
• Product Overview: Recombinant Human IL27 protein(NP_005746.2 tag and NP_663634.2)(Met1-Lys229 tag and Phe29-Pro243), fused with His tag, was expressed in CHO.

Specification

• Source: CHO
• Species: Human
• Tag: His
• Form: Lyophilized from sterile 20mM MOPS,150mM NaCl, 0.05% CHAPS, PH7.0Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized human IL27-His at 10 μg/ml (100 μl/well) can bind human IL27RA-Fc, The EC50 of human IL27RA-Fc is 0.2-0.46 μg/ml.2. Measured in antiviral assays using HepG2 cells infected with vesicular stomatitisvirus(VSV). The ED50 for this effect is typically 23-92 ng/mL.
• Molecular Mass: The recombinant human IL27 complex (IL27B & IL27A) consists of 450 amino acids after removal of the signal peptide and has a calculated molecular mass of 56 KDa as estimated in SDS-PAGE under reducing conditions.
• Proteinlength: Met1-Lys229&Phe29-Pro243
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Protein length: 1-229;29-243 a.a.

Gene Information

• Gene Name: IL27 interleukin 27 [ Homo sapiens ]
• Official Symbol: IL27
• Synonyms: IL27; interleukin 27
• Gene ID: 144841