Active Recombinant Human IL21 Protein (aa 32-162), N-Fc-tagged

• Cat.No.: IL21-01H
• Product Overview: The extracellular domain of human IL-21 (aa 32-162) is fused to the N-terminus of the Fc region of a mutant human IgG4 was expressed in CHO.

Specification

• Description: This gene encodes a member of the common-gamma chain family of cytokines with immunoregulatory activity. The encoded protein plays a role in both the innate and adaptive immune responses by inducing the differentiation, proliferation and activity of multiple target cells including macrophages, natural killer cells, B cells and cytotoxic T cells. Dysregulation of this gene plays a role in multiple immune-mediated diseases including lupus, psoriasis and chronic inflammatory diseases. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Source: CHO
• Species: Human
• Tag: Fc
• Bio-activity: Measured in a cell proliferation assay using N1186 human T cells.
• Molecular Mass: ~42 kDa (SDS-PAGE)
• Protein length: 32-162
• Endotoxin: <5 EU/mg protein (LAL test; Lonza).
• Purity: ≥95% (SDS-PAGE)
• Stability: Stable for at least 1 year after receipt when stored at -20 centigrade.
  Working aliquots are stable for up to 3 months when stored at -20 centigrade.
• Storage: Short Term Storage: +4 centigrade
  Long Term Storage: -20 centigrade
• Storage Buffer: Lyophilized from 0.2 μm-filtered solution in PBS.
• Reconstitution: Reconstitute 50 μg vial in 50 μL sterile water.
  Add 1 × PBS to the desired protein concentration.
• Handling: Avoid freeze/thaw cycles.
  Centrifuge lyophilized vial before opening and reconstitution.
• Shipping: BLUE ICE

Gene Information

• Gene Name: IL21 interleukin 21 [ Homo sapiens (human) ]
• Official Symbol: IL21
• Synonyms: IL21; interleukin 21; Za11; IL-21; CVID11; interleukin-21
• Gene ID: 59067
• mRNA Refseq: NM_021803
• Protein Refseq: NP_068575
• MIM: 605384
• UniProt ID: Q9HBE4