Active Recombinant Human IL1RAP protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: IL1RAP-0264H
• Product Overview: Biotinylated Recombinant Human IL1RAP protein(Ser21-Glu359), fused with Fc and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Fc
• Protein Length: 21-359 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Human Anti-IL1RAP Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human IL-1 RAcP / IL-1 R3,Fc,Avitag with a linear range of 0.5-16 ng/mL.Immobilized Biotinylated Human IL-1 RAcP / IL-1 R3,Fc,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human Anti-IL1RAP Human IgG1 with a linear range of 0.2-4 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag)The protein has a calculated MW of 66 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ser21-Glu359
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL1RAP interleukin 1 receptor accessory protein [ Homo sapiens ]
• Official Symbol: IL1RAP
• Synonyms: IL1RAP; interleukin 1 receptor accessory protein; interleukin-1 receptor accessory protein; C3orf13; IL 1RAcP; IL1R3; IL-1R3; interleukin-1 receptor 3; IL-1 receptor accessory protein; interleukin-1 receptor accessory protein beta; IL-1RAcP; FLJ37788
• Gene ID: 3556
• mRNA Refseq: NM_001167928
• Protein Refseq: NP_001161400
• MIM: 602626
• UniProt ID: Q9NPH3