Active Recombinant Human IL13 protein, Fc-tagged

• Cat.No.: IL13-365H
• Product Overview: Human IL-13, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Asn 146 (Accession # NP_002179.2).

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Leu 25 - Asn 146
• Description: Interleukin 13 (IL13) is also known as ALRH, BHR1and P600, is a single-chain glycosylated polypeptide, and is a cytokine critical in regulating inflammatory and immune responses. IL13 is secreted by many cell types, but especially by T helper type 2 (Th2) cells. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Rα) and at least one of two known IL-13-specific binding chains. The functions of IL-13 overlap considerably with those of IL-4, especially with regard to changes induced on hematopoietic cells, but these effects are probably less important given the more potent role of IL-4. IL-13 induces matrix metalloproteinases (MMPs) as part of a mechanism that protects against excessive allergic inflammation that predisposes to asphyxiation. IL-13 induces many features of allergic lung disease, including airway hyperresponsiveness, goblet cell metaplasia and mucus hypersecretion, which all contribute to airway obstruction.
• Predicted N Terminal: Gly
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris,100 mM Glycine, pH7.5, 10% trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human IL-13RA1, His Tag at 10 μg/mL (100 μL/well) can bind Human IL-13, Fc Tag with a linear range of 0.256-10 μg/mL. Measured by its binding ability in a functional ELISA. Immobilized Human IL-13, Fc Tag at 10 μg/mL (100 μL/well) can bind Anti IL-13 MAb, Human IgG4 with a linear range of 0.8-25 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 40.2 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Storage buffer: 50 mM Tris,100 mM Glycine, pH7.5
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Shipping: Powder,RT

Gene Information

• Gene Name: IL13 interleukin 13 [ Homo sapiens (human) ]
• Official Symbol: IL13
• Synonyms: P600; IL-13; interleukin-13
• Gene ID: 3596
• mRNA Refseq: NM_002188.2
• Protein Refseq: NP_002179.2
• UniProt ID: P35225