Active Recombinant Human IL13

• Cat.No.: IL13-1251H
• Product Overview: Recombinant human IL-13 produced in E.coli is a single, non-glycosylated, polypeptide chain containing 113 amino acids with two pairs of disulfate bond and having a molecular mass of approximately 12.3kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: IL-13 is an immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils. IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four α helix configuration. Mature human IL-13 shares 57%, 59%, and 94% amino acid sequence identity with mouse, rat, and rhesus IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat. IL13 has diverse activities on numerous cell types. On macrophages, IL13 suppresses the production of proinflammatory cytokines and other cytotoxic substances. On B cells, IL13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while down-regulating IL-1 and TNFα production by fibroblasts and endothelial cells. IL-13R-α2 regulates the bioavailability of both IL-13 and IL-4 and is overexpressed in glioma and several bronchial pathologies. Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13R-α2.
• Form: Lyophilized from a 0.2μm filtered solution in PBS containing 0.1% HSA, pH7.4.
• Bio-activity: Measured in a cell proliferation assay using TF-1 cell line. The specific activity shall be not less than 1 x 10^6 IU/mg.
• Endotoxin: ≤1EU/μg, determined by the LAL method.
• Purity: ≥97%, as determined by SDS-PAGE and HPLC method.
• Usage: FOR RESEARCH ONLY
• Storage: Lyophilized samples are stable for greater than six months from date of receipt at -20 centigrade to -70 centigrade. The reconstituted samples can be stored under sterile conditions at 2-8 centigrade for one month or at -20 centigrade to -70 centigrade for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of not less than 100μg/ml.

Gene Information

• Gene Name: IL13 interleukin 13 [ Homo sapiens ]
• Official Symbol: IL13
• Synonyms: P600; IL-13; interleukin-13
• Gene ID: 3596
• mRNA Refseq: NM_002188
• Protein Refseq: NP_002179
• MIM: 147683
• UniProt ID: P35225
• Chromosome Location: 5q31
• Pathway: Asthma, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Fc epsilon RI signaling pathway, organism-specific biosystem
• Function: cytokine activity; interleukin-13 receptor binding; protein binding