Active Recombinant Human IGFBP4, Met-tagged

• Cat.No.: IGFBP4-191H
• Product Overview: Recombinant Human IGFBP4 (Accession # AAA62670) Asp22-Glu258, fused with an N-terminal Met, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Predicted N Terminal: Met
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083.The ED50 for this effect is typically 0.03-0.09 µg/mL in the presence of 14 ng/mL recombinant human IGF-II.
• Molecular Mass: Recombinant Human IGFBP4, Met-tagged has a calculated MW of 26 kDa. In SDS-PAGE migrates as 32 kDa, reducing conditions.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Protein length: 22-258 a.a.

Gene Information

• Gene Name: IGFBP4 insulin-like growth factor binding protein 4 [ Homo sapiens ]
• Official Symbol: IGFBP4
• Synonyms: IGFBP4; insulin-like growth factor binding protein 4; insulin like growth factor binding protein 4; insulin-like growth factor-binding protein 4; BP 4; HT29 IGFBP; IBP4; IGF binding protein 4; IGFBP 4; IBP-4; IGF-binding protein 4; BP-4; IGFBP-4; HT29-IGFBP
• Gene ID: 3487
• mRNA Refseq: NM_001552
• Protein Refseq: NP_001543
• MIM: 146733
• UniProt ID: P22692
• Chromosome Location: 17q12-q21.1