Active Recombinant Human IGFBP-4, Carrier Free

• Cat.No.: IGFBP4-8H
• Product Overview: Mature IGFBP-4 (Kiefer, M. et al., 1991, J. Biol. Chem. 266:9043 - 9049) (aa residues Asp 22 - Glu 258) was fused to the signal peptide of CD33 and the chimera was expressed in a mouse myeloma cell line, NS0.

Specification

• Description: Human IGF binding protein 4 (IGFBP-4) was isolated from human plasma based on its ability to bind immobilized IGF-I. Human IGFBP-4 cDNA encodes a 258 amino acid (aa) residue precursor protein with a predicted 21 aa residue signal peptide that is pr centigradeessed to generate the 237 aa residue mature human IGFBP-4. The human IGFBP-4 contains a potential N-linked glycosylation site and shares approximately 90% amino acid sequence identity with both the mouse and rat IGFBP-4. According to the nomenclature of IGFBPs defined at the 4th International Symposium of IGFs (1997, Tokyo), six high-affinity IGF binding proteins (IGFBP-1, -2, -3, -4, -5, -6), and four IGFBP-related proteins (IGFBPr-1, - 2, -3, -4) have now been identified. All IGFBPs have a high cysteine content and share conserved cysteine residues that are clustered in the amino- and carboxy-terminal thirds of the molecule.
• Source: Mammalian cells
• Species: Human
• Form: Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4.
• Bio-activity: Measured by its ability to inhibit the biological activity of rhIGF-I or rhIGF-II on MCF-7 cells (Karey, K.P. et al., 1988, Cancer Research 48:4083). The ED50 for this effect is typically 0.03 - 0.09 μg/mL in the presence of 14 ng/mL rhIGF-II.
• Molecular Mass: Met 17 from the CD33 signal peptide was retained in the recombinant mature human IGFBP-4. The 238 amino acid residue recombinant mature human IGFBP-4 has a calculated molecular mass of approximately 26 kDa. As a result of glycosylation, the recombinant protein migrates as a 32 kDa and 25 kDa protein under reducing and non-reducing conditions, respectively.
• Endotoxin: < 1.0 eu per 1 μg of the cytokine as determined by the lal
• Purity: >97%, as determined by SDS-PAGE and visualized by silver stain.
• Usage: FOR RESEARCH USE ONLY
• Quality Control Test: Lyophilized samples are stable for up to six months at -20 centigrade to -70 centigrade. Upon reconstitution, this cytokine, in the presence of a carrier protein, can be stored under sterile conditions at 2 - 8 centigrade for one month or at -20 centigrade to -70 centigrade in a manual defrost freezer for three months without detectable loss of activity.
• Reconstitution: It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a st centigradek solution of no less than 25 μg/mL.
• Warning: Avoid repeated freeze-thaw cycles.
• Tag: Non
• Protein length: 22-258 a.a.

Gene Information

• Gene Name: IGFBP4 insulin-like growth factor binding protein 4 [ Homo sapiens ]
• Official Symbol: IGFBP4
• Synonyms: IGFBP4; insulin-like growth factor binding protein 4; insulin like growth factor binding protein 4; insulin-like growth factor-binding protein 4; BP 4; HT29 IGFBP; IBP4; IGF binding protein 4; IGFBP 4; IBP-4; IGF-binding protein 4; BP-4; IGFBP-4; HT29-IGFBP
• Gene ID: 3487
• mRNA Refseq: NM_001552
• Protein Refseq: NP_001543
• MIM: 146733
• UniProt ID: P22692
• Chromosome Location: 17q12-q21.1
• Pathway: Diabetes pathways, organism-specific biosystem; Disease, organism-specific biosystem; Myometrial Relaxation and Contraction Pathways, organism-specific biosystem; Regulation of Insulin-like Growth Factor (IGF) Activity by Insulin-like Growth Factor Binding Proteins (IGFBPs), organism-specific biosystem; Wnt signaling network, organism-specific biosystem
• Function: insulin-like growth factor binding