Active Recombinant Human IGFBP3, Met-tagged

• Cat.No.: IGFBP3-489H
• Product Overview: Recombinant Human IGFBP3 (Accession # CAA46087) Gly28-Lys291, fused with an N-terminal Met, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Human
• Source: Mammalian Cells
• Protein Length: 28-291 a.a.
• Predicted N Terminal: Met
• Form: Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA.
• Bio-activity: Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083.The ED50 for this effect is typically 0.05 - 0.15 µg/mL in the presence of 14 ng/mL rhIGF-II.
• Molecular Mass: Recombinant Human IGFBP3, Met-tagged has a calculated MW of 29 kDa. In SDS-PAGE migrates as 41 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: IGFBP3 insulin-like growth factor binding protein 3 [ Homo sapiens ]
• Official Symbol: IGFBP3
• Synonyms: IGFBP3; insulin-like growth factor binding protein 3; insulin-like growth factor-binding protein 3; acid stable subunit of the 140 K IGF complex; binding protein 29; binding protein 53; BP 53; growth hormone dependent binding protein; IBP3; IGF binding protein 3; IBP-3; IGFBP-3; IGF-binding protein 3; growth hormone-dependent binding protein; BP-53
• Gene ID: 3486
• mRNA Refseq: NM_000598
• Protein Refseq: NP_000589
• MIM: 146732
• UniProt ID: P17936
• Chromosome Location: 7p13-p12