Active Recombinant Human IFNG Protein

• Cat.No.: IFNG-084H
• Product Overview: The mature recombinant human IFN- γ protein (24-166aa) without tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Protein Length: 24-166 a.a.
• Description: This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases.
• Bio-activity: Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.15-0.75 ng/mL.
  The specific activity of Recombinant Human IFN-gamma is approximately 2 x 104 IU/μg, which is calibrated against human IFN-gamma Standard (NIBSC code: 87/586).
• Molecular Mass: 17 kDa
• Product-Related Proteins: 24-166
• Endotoxin: < 0.1 EU/μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: At -80 centigrade. Avoid repeated freeze-thaw cycles.
• Storage Buffer: PBS

Gene Information

• Gene Name: IFNG interferon gamma [ Homo sapiens (human) ]
• Official Symbol: IFNG
• Synonyms: IFNG; interferon gamma; IFG; IFI; IMD69; interferon gamma; Names; IFN-gamma; immune interferon
• Gene ID: 3458
• mRNA Refseq: NM_000619
• Protein Refseq: NP_000610
• MIM: 147570
• UniProt ID: P01579