Active Recombinant Human IFNAR2 protein(Met1-Lys243), hFc-tagged

• Cat.No.: IFNAR2-157H
• Product Overview: Recombinant Human IFNAR2 protein(NP_997468.1)(Met1-Lys243), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Lys243
• similar: IFNAR2
• Alias: IFNAR2; interferon (alpha, beta and omega) receptor 2; IFNABR; interferon alpha/beta receptor 2; IFN-R-2; IFN-alpha binding protein; IFN-alpha/beta receptor 2; type I interferon receptor 2; interferon alpha binding protein; human interferon alpha/beta receptor; interferon-alpha/beta receptor beta chain; IFN-R; IFNARB; IFN-alpha-REC
• Conjugate: C-hFc
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bioactivity: 1. Measured by its ability to inhibit rh IFNβ mediated protection of WISH Human amnion cells infected with vesicular stomatitis virus (VSV) to viral lysis. The EC50 for this effect is typically 0.2-1.2 μg/mL. 2. Measured by its binding ability in a functional ELISA. Immobilized PPICZa-IFNA2 at 10 μg/mL (100 μL/well) can bind IFNaR2-Fc, the EC50 of IFNaR2-Fc is 60-200 ng/mL.
• MolecularMass: The recombinant human IFNAR2/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 458 amino acids and predicts a molecular mass of 51.8 kDa. As a result of glycosylation, the apparent molecular mass of rh IFNAR2/Fc monomer is approximately 65-75 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• GeneName: IFNAR2 interferon (alpha, beta and omega) receptor 2 [ Homo sapiens ]
• GeneNameWeblink: 3455
• OfficialSymbol: IFNAR2
• OfficialSymbolWeblink: 3455
• GeneID: 3455
• GeneIDWeblink: 3455
• mRNARefseq: NM_000874
• mRNARefseqWeblink: NM_000874.3
• ProteinRefseqWeblink: NP_000865.2
• MIMWeblink: 602376
• UniProtID: P48551
• UniProtIDWeblink: P48551

Gene Information

• Synonyms: IFNAR2; interferon (alpha, beta and omega) receptor 2; IFNABR; interferon alpha/beta receptor 2; IFN-R-2; IFN-alpha binding protein; IFN-alpha/beta receptor 2; type I interferon receptor 2; interferon alpha binding protein; human interferon alpha/beta receptor; interferon-alpha/beta receptor beta chain; IFN-R; IFNARB; IFN-alpha-REC
• MIM: 602376