Active Recombinant Human IDO1, His-tagged

• Cat.No.: IDO1-294H
• Product Overview: Human IDO (aa 1-403) is fused at the C-terminus to a His-tag.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-403 a.a.
• Description: IDO catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway. Proinflammatory mediators, such as endotoxin and IFN-γ induce the expression of IDO in several tissues. IDO-dependent suppression of T cell responses might function as natural immunoregulatory mechanism. Physiological IDO activity has been implicated in T cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders.
• Form: Liquid. 0.2μm-filtered solution in 50mM TRIS-Cl, pH 7.4, containing 1mM EDTA.
• Bio-activity: Specific Activity: >100’000U/mg protein with L-tryptophan as substrate. One unit is defined as the amount of enzyme that produces 1nmol of kynurenine per hour.
• Molecular Mass: ~40kDa (SDS-PAGE)
• Purity: ≥90% (SDS-PAGE)
• Stability: Working aliquots are stable for up to 3 months when stored at -20°C.
• Storage: Short Term Storage: +4°C; Long Term Storage: -20°C. After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
• Concentration: 1mg/ml

Gene Information

• Gene Name: IDO1 indoleamine 2,3-dioxygenase 1 [ Homo sapiens ]
• Official Symbol: IDO1
• Synonyms: IDO1; indoleamine 2,3-dioxygenase 1; IDO, INDO, indoleamine pyrrole 2,3 dioxygenase; indole 2,3-dioxygenase; indolamine 2,3 dioxygenase; indoleamine-pyrrole 2,3-dioxygenase; IDO; INDO; IDO-1
• Gene ID: 3620
• mRNA Refseq: NM_002164
• Protein Refseq: NP_002155
• MIM: 147435
• UniProt ID: P14902
• Chromosome Location: 8p12-p11
• Pathway: African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; Tryptophan catabolism, organism-specific biosystem; Tryptophan metabolism, organism-specific biosystem
• Function: electron carrier activity; heme binding; indoleamine 2,3-dioxygenase activity; metal ion binding; oxidoreductase activity; tryptophan 2,3-dioxygenase activity