Active Recombinant Human IDO1 protein, His-tagged

• Cat.No.: IDO1-008H
• Product Overview: Active recombinant human N-terminal His-tagged IDO1 was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 2-403 (full length)
• Description: This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.
• Form: 40 mM Tris-HCl, pH 8.0, with 110 mM sodium chloride, 2.2 mM potassium chloride, and 20% glycerol.
• Bio-activity: IDO1 activity is measured by incubating with L-Trp substrate. After a three hour roomtemperature incubation, activity is determined by measuring the absorption of reaction product at λ = 320-325 nm.
• Molecular Mass: 46 kDa
• Purity: ≥90% estimated by SDS-PAGE
• Applications: Enzyme Activity
• Stability: ≥ 6 months
• Storage: -80 centigrade
• Shipping: Dry ice in continental US; may vary elsewhere

Gene Information

• Gene Name: IDO1 indoleamine 2,3-dioxygenase 1 [ Homo sapiens (human) ]
• Official Symbol: IDO1
• Synonyms: Indoleamine 2,3-dioxygenase 1 Indoleamine-pyrrole 2,3-dioxygenase
• Gene ID: 3620
• mRNA Refseq: NM_002164
• Protein Refseq: NP_002155
• MIM: 147435
• UniProt ID: P14902