Active Recombinant Human HMGB1 protein, His-tagged

• Cat.No.: HMGB1-2564H
• Product Overview: Active Recombinant Human HMGB1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Glu215) of human HMGB1 (Accession #NP_002119.1) fused with a 6×His tag at the C-terminus.

Specification

• Product Overview: Active Recombinant Human HMGB1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Met1-Glu215) of human HMGB1 (Accession #NP_002119.1) fused with a 6×His tag at the C-terminus.
• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human HMGB1 at 2μg/mL (100 μL/well) can bind Human AGER/RAGE with a linear range of 0.1-42.8 ng/mL.
• Molecular Mass: 30 kDa
• Protein length: Met1-Glu215
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: > 97% by SDS-PAGE.
• Storage: Store the lyophilized protein at -20°C to -80 °C for long term.
  After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
• Reconstitution: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

Gene Information

• Gene Name: HMGB1 high mobility group box 1 [ Homo sapiens ]
• Official Symbol: HMGB1
• Synonyms: HMGB1; high mobility group box 1; high mobility group (nonhistone chromosomal) protein 1 , high mobility group box 1 , HMG1; high mobility group protein B1; Amphoterin; DKFZp686A04236; high mobility group protein 1; HMG3; SBP 1; Sulfoglucuronyl carbohydrate binding protein; HMG-1; high-mobility group box 1; high-mobility group (nonhistone chromosomal) protein 1; HMG1; SBP-1
• Gene ID: 3146
• mRNA Refseq: NM_002128
• Protein Refseq: NP_002119
• MIM: 163905
• UniProt ID: P09429