Active Recombinant Human HMGB1 Protein, His-tagged

• Cat.No.: HMGB1-526H
• Product Overview: Recombinant Human HMGB1 Protein (P09429) (Lys57-Asp158) was expressed in E. coli with a polyhistidine tag at the N-terminus.

Specification

• Product Overview: Recombinant Human HMGB1 Protein (P09429) (Lys57-Asp158) was expressed in E. coli with a polyhistidine tag at the N-terminus.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized Powder
• Bio-activity: Immobilized human His-HMGB1 at 10 μg/ml (100 μl/well) can bind AGER-Fc with a linear range of 0.31-2.5 μg/ml.
• Molecular Mass: The recombinant human HMGB1 consists of 117 amino acids and predicts a molecular mass of 13.6 KDa. It migrates as an approximately 15 KDa band in SDS-PAGE under reducing conditions.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade.
• Storage Buffer: Lyophilized from sterile PBS, 20% Glycerol, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Protein length: 57-158 a.a.

Gene Information

• Gene Name: HMGB1 high mobility group box 1 [ Homo sapiens ]
• Official Symbol: HMGB1
• Synonyms: HMG1; HMG3; HMG-1; SBP-1
• Gene ID: 3146
• mRNA Refseq: NM_002128
• Protein Refseq: NP_002119
• MIM: 163905
• UniProt ID: P09429