Active Recombinant Human HIPK4 Protein, GST-tagged

• Cat.No.: HIPK4-4767H
• Product Overview: Human HIPK4 (NP_653286.2, 1 a.a. - 616 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Description: This gene encodes a member of the homeodomain interacting protein kinase (HIPK) family of proteins. While other members of this family are found throughout vertebrates, this member is present only in mammals. Compared to other members of this family, the encoded protein lacks a nuclear localization signal and a C-terminal autoinhibitory domain. The encoded protein exhibits kinase activity and may phosphorylate the tumor suppressor protein p53. [provided by RefSeq, Jul 2016]
• Form: Liquid
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000.
  Substrate: DYRKtide-F.
  ATP: 100 μM.
• Molecular Mass: 96 kDa
• Purity: 70 % by SDS-PAGE/CBB staining
• Applications: Functional Study
  SDS-PAGE
• Storage: Store at -8 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)

Gene Information

• Gene Name: HIPK4 homeodomain interacting protein kinase 4 [ Homo sapiens ]
• Official Symbol: HIPK4
• Synonyms: HIPK4; homeodomain interacting protein kinase 4; homeodomain-interacting protein kinase 4; FLJ32818
• Gene ID: 147746
• mRNA Refseq: NM_144685
• Protein Refseq: NP_653286
• MIM: 611712
• UniProt ID: Q8NE63