Active Recombinant Human Heparan Sulfate (glucosamine) 3-O-Sulfotransferase 4, His-tagged

• Cat.No.: HS3ST4-1971H
• Product Overview: RecombinantHuman HS3ST4 protein was expressed in Chinese HamsterOvary cell line. Gly184-Lys456, with an N-terminal 6-Histag.

Specification

• Cat. No.: HS3ST4-1971H
• Description: Heparansulfate is a highly sulfated polysaccharide that can be found on the cellsurface and within the extracellular matrix. It is typically covalently attachedto the protein core of proteoglycans, such as syndecans and glypicans.Heparin, on the other hand, can be considered as a highly sulfated version ofheparan sulfate that is predominantly found in mast cells. Both heparin andheparan sulfate contain disaccharide repeats of uronic acid and N-acetylglucosamineand are modified by the same sulfotransferases. The uronic acid residues canbe sulfated at the 2-O position by heparin sulfate 2-O sulfotransferase(HS2ST). The N-acetylglucosamine residues can be sulfated at the N, 3-O, and6-O positions by N-deacetylase/ N-sulfotransferases (NDSTs), heparan sulfate3-O sulfotransferases (HS3STs) and heparan sulfate 6-O sulfotransferases(HS6STs) respectively. There are seven HS3STs in the human genome.
• Source: Chinese HamsterOvary cell line, CHO-derived.
• Form: Supplied as a 0.2 μmfiltered solution in Tris, NaCl and Glycerol.
• N-terminalSequence: HHHHHHGGAV
• MolecularWeight: 32 kDa
• Activity: Measuredby its ability to transfer sulfate from PAPS to heparan sulfate. The specificactivity is > 5 pmoles/min/μg, as measured under the described conditions.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 35-43kDa, reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by Colloidal CoomassieBlue stain at 5 ug per lane.
• Storage: Use amanual defrost freezer and avoid repeated freezethaw cycles. 6 monthsfrom date of receipt, -20 to -70°C as supplied. 3 months,-20to-70°C under sterile conditions after opening.
• Protein length: Gly184-Lys456

Gene Information

• Gene Name: HS3ST4 heparan sulfate(glucosamine) 3-O-sulfotransferase 4 [ Homo sapiens ]
• Official Symbol: HS3ST4
• Synonyms: HS3ST4; heparansulfate (glucosamine) 3-O-sulfotransferase 4; 30ST4;3OST4; 3-OST-4; h3-OST-4; heparan sulfate glucosamine3-O-sulfotransferase 4; heparan sulfate 3-O-sulfotransferase 4; heparansulfate D-glucosaminyl 3-O-sulfotransferase 4; EC 2.8.2.23
• Gene ID: 9951
• mRNA Refseq: NM_006040
• Protein Refseq: NP_006031
• MIM: 604059
• UniProt ID: Q9Y661
• Chromosome Location: 16p11.2
• Function: [heparansulfate]-glucosamine 3-sulfotransferase 1 activity; sulfotransferase activity;transferase activity