Active Recombinant Human HDAC5 Protein, His-tagged

• Cat.No.: HDAC5-96H
• Product Overview: Recombinant Human HDAC5 fused with His tag was expressed in Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 476 amino acids
• Description: HDAC5 is a member of the histone deacetylase family. It is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC5 possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes.
• Form: The HDAC5 solution contains 20 mM Tris-HCl buffer (pH 8.0), 300 mM NaCl and 20% Glycerol. Frozen solution.
• Bio-activity: > 10000 pmol/min/μg. Assay condition: 50 mM Tris-HCl, pH8.0, 136 mM NaCl, 2.8 mM KCl, 1 mM MgCl2, 0.5 mg/ml BSA, 0.005% Tween-20, 20 M AMC-RHKKAc and HDAC5. HDAC reaction was done for 30 min at 37 centigrade followed by treatment with equivoluminal HDAC developer buffer (40 μM Trypsin, 40 μM CaCl2 and 20 μM Trichostatin A for ~60 min at RT.
• Molecular Mass: 52.2 kDa, observed by reducing SDS-PAGE.
• AA Sequence: MGRTQSSPAA PGGMKSPPDQ PVKHLFTTGV VYDTFMLKHQ
  CMCGNTHVHP EHAGRIQSIW SRLQETGLLS KCERIRGRKA
  TLDEIQTVHS EYHTLLYGTS PLNRQKLDSK KLLGPISQKM
  YAVLPCGGIG VDSDTVWNEM HSSSAVRMAV GCLLELAFKV
  AAGELKNGFA IIRPPGHHAE ESTAMGFCFF NSVAITAKLL
  QQKLNVGKVL IVDWDIHHGN GTQQAFYNDP SVLYISLHRY
  DNGNFFPGSG APEEVGGGPG VGYNVNVAWT GGVDPPIGDV
  EYLTAFRTVV MPIAHEFSPD VVLVSAGFDA VEGHLSPLGG
  YSVTARCFGH LTRQLMTLAG GRVVLALEGG HDLTAICDAS
  EACVSALLSV ELQPLDEAVL QQKPNINAVA TLEKVIEIQS
  KHWSCVQKFA AGLGRSLREA QAGETEEAET VSAMALLSVG
  AEQAQAAAAR EHSPRPAEEP MEQEPALHHH HHHHHHH
• Endotoxin: < 1 EU/μg, determined by LAL method.
• Purity: > 90% by SDS-PAGE analysis.
• Storage: HDAC5 remains stable up to 6 months at -80 centigrade from date of receipt. For maximum recovery of product, centrifuge original vial prior to removing the cap. Aliquot to avoid repeated thawing and freezing.

Gene Information

• Gene Name: HDAC5 histone deacetylase 5 [ Homo sapiens ]
• Official Symbol: HDAC5
• Synonyms: HDAC5; histone deacetylase 5; FLJ90614; KIAA0600; NY CO 9; antigen NY-CO-9; HD5; NY-CO-9
• Gene ID: 10014
• mRNA Refseq: NM_005474
• Protein Refseq: NP_005465
• MIM: 605315
• UniProt ID: Q9UQL6