Active Recombinant Human HAO1 Protein, His-tagged

• Cat.No.: HAO1-001H
• Product Overview: Recombinant human HAO1 protein(1-370aa), fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-370 a.a.
• Description: HAO1, also known as glycolate oxidase, is a member of the superfamily of the alpha hydroxy acid oxidases (HAO) enzymes. It catalyzes the FMN mediated oxidation of glycolate to glyoxylate and glyoxylate to oxalate with reduction of oxygen to hydrogen peroxide. It is most highly expressed in liver and pancreas and is most active on twocarbon substrates such as glycolate. Recently, it has been identified as a major contributor to hyperoxaluria, a disorder in which large deposits of calcium oxalate form kidney stones.
• Form: Liquid
• Bio-activity: Specific activity is > 3,000 pmol/min/μg, and defined as the amount of enzyme that oxidize glyoxylate at pH 8.0 at 25 centigrade.
• Molecular Mass: 45.0 kDa
• AA Sequence: MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMLPRLICINDYEQHAKSVLPKSIYDYYRSGANDEETLADNIAAFSRWKLYPRMLRNVAETDLSTSVLGQRVSMPICVGATAMQRMAHVDGELATVRACQSLGTGMMLSSWATSSIEEVAEAGPEALRWLQLYIYKDREVTKKLVRQAEKMGYKAIFVTVDTPYLGNRLDDVRNRFKLPPQLRMKNFETSTLSFSPEENFGDDSGLAAYVAKAIDPSISWEDIKWLRRLTSLPIVAKGILRGDDAREAVKHGLNGILVSNHGARQLDGVPATIDVLPEIVEAVEGKVEVFLDGGVRKGTDVLKALALGAKAVFVGRPIVWGLAFQGEKGVQDVLEILKEEFRLAMALSGCQNVKVIDKTLVRKNPLAVSKI
• Purity: > 95% by SDS-PAGE
• Notes: For research use only. This product is not intended or approved for human, diagnostics or veterin.
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 or -70 centigrade.
  Avoid repeated freezing and thawing cycles.
• Concentration: 1.0 mg/mL
• Storage Buffer: 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.5M NaCl.

Gene Information

• Gene Name: HAO1 hydroxyacid oxidase 1 [ Homo sapiens (human) ]
• Official Symbol: HAO1
• Synonyms: GOX (glycolate oxidase); GOX1; HAOX1; Hydroxyacid oxidase 1; GOX; hydroxyacid oxidase 1; (S)-2-hydroxy-acid oxidase; glycolate oxidase 1; hydroxyacid oxidase (glycolate oxidase) 1; EC 1.1.3.15
• Gene ID: 54363
• mRNA Refseq: NM_017545
• Protein Refseq: NP_060015
• MIM: 605023
• UniProt ID: Q9UJM8