Active Recombinant Human HAO1 Protein, His-tagged

• Cat.No.: HAO1-001H
• Product Overview: Recombinant human HAO1 protein(1-370aa), fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Description: HAO1, also known as glycolate oxidase, is a member of the superfamily of the alpha hydroxy acid oxidases (HAO) enzymes. It catalyzes the FMN mediated oxidation of glycolate to glyoxylate and glyoxylate to oxalate with reduction of oxygen to hydrogen peroxide. It is most highly expressed in liver and pancreas and is most active on twocarbon substrates such as glycolate. Recently, it has been identified as a major contributor to hyperoxaluria, a disorder in which large deposits of calcium oxalate form kidney stones.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid
• Bio-activity: Specific activity is > 3,000 pmol/min/μg, and defined as the amount of enzyme that oxidize glyoxylate at pH 8.0 at 25 centigrade.
• Molecular Mass: 45.0 kDa
• AA Sequence: MRGSHHHHHHGMASMTGGQQMGRDL YDDDDKDRWGSMLPRLICINDYEQH AKSVLPKSIYDYYRSGANDEETLAD NIAAFSRWKLYPRMLRNVAETDLST SVLGQRVSMPICVGATAMQRMAHVD GELATVRACQSLGTGMMLSSWATSS IEEVAEAGPEALRWLQLYIYKDREV TKKLVRQAEKMGYKAIFVTVDTPYL GNRLDDVRNRFKLPPQLRMKNFETS TLSFSPEENFGDDSGLAAYVAKAID PSISWEDIKWLRRLTSLPIVAKGIL RGDDAREAVKHGLNGILVSNHGARQ LDGVPATIDVLPEIVEAVEGKVEVF LDGGVRKGTDVLKALALGAKAVFVG RPIVWGLAFQGEKGVQDVLEILKEE FRLAMALSGCQNVKVIDKTLVRKNP LAVSKI
• Purity: > 95% by SDS-PAGE
• Notes: For research use only. This product is not intended or approved for human, diagnostics or veterin.
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 or -70 centigrade.
  Avoid repeated freezing and thawing cycles.
• Concentration: 1.0 mg/mL
• Storage Buffer: 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.5M NaCl.
• Protein length: 1-370

Gene Information

• Gene Name: HAO1 hydroxyacid oxidase 1 [ Homo sapiens (human) ]
• Official Symbol: HAO1
• Synonyms: GOX (glycolate oxidase); GOX1; HAOX1; Hydroxyacid oxidase 1; GOX; hydroxyacid oxidase 1; (S)-2-hydroxy-acid oxidase; glycolate oxidase 1; hydroxyacid oxidase (glycolate oxidase) 1; EC 1.1.3.15
• Gene ID: 54363
• mRNA Refseq: NM_017545
• Protein Refseq: NP_060015
• MIM: 605023
• UniProt ID: Q9UJM8

Reviews

05/09/2022

Worked well in experiments.

11/28/2020

Great for academic research.

01/28/2020

Very satisfied, will buy again.

Q&As

How is HAO1 implicated in metabolic disorders?

Dysregulation of HAO1 is linked to metabolic disorders and fatty liver disease.

Are there existing or potential therapeutic strategies targeting HAO1?

Therapeutic approaches may include modulation of HAO1 activity or expression.

How does HAO1 contribute to fatty acid metabolism?

It oxidizes specific fatty acids, contributing to their breakdown.

What are the key biological functions of HAO1?

HAO1 is involved in peroxisomal fatty acid oxidation, crucial for lipid metabolism.

What are the potential consequences of HAO1 overexpression?

HAO1 overexpression may lead to oxidative stress and liver dysfunction.

What role does HAO1 play in liver function?

HAO1 supports liver function by participating in lipid catabolism.

How is HAO1 activity regulated in cells?

Cellular regulation of HAO1 involves transcriptional and post-translational mechanisms.