Recombinant Human HAO1, TraxA/His-tagged

• Cat.No.: HAO1-99H
• Product Overview: Recombinant Human Hydroxyacid Oxidase 1/HAO1 isproduced by our E. coli expression system. The target protein is expressed with sequence (Met1-Ile370) of Human HAO1 fused with a TraxA/His tag at the N-terminus.

Specification

• Description: Hydroxyacid Oxidase 1 (HAO1) is an enzyme that belongs to the FMN-Dependent α-Hydroxy Acid Dehydrogenase family. HAO1 contains 1 FMN Hydroxy Ccid Dehydrogenase domain. HAO1 is expressed primarily in the liver and pancreas. This protein has 2-Hydroxyacid Oxidase activity. Most HAO1 is active on the 2-Carbon substrate Glycolate, but it can also be active on 2-Hydroxy fatty acids, with higher activity towards 2-Hydroxy Palmitate and 2-Hydroxy Octanoate.
• Source: E. coli
• Species: Human
• Tag: His&TrxA
• Form: Supplied as a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2
• AA Sequence: MSDKIIHLTDDSFDTDVLKADGAIL VDFWAEWCGPCKMIAPILDEIADEY QGKLTVAKLNIDQNP GTAPKYGIRGIPTLLLFKNGEVAAT KVGALSKGQLKEFLDANLAGSGSGH MHHHHHHSSGLVPRG SGMKETAAAKFERQHMDSPDLGTDD DDKAMADIGSMLPRLICINDYEQHA KSVLPKSIYDYYRSG ANDEETLADNIAAFSRWKLYPRMLR NVAETDLSTSVLGQRVSMPICVGAT AMQRMAHVDGELATV RACQSLGTGMMLSSWATSSIEEVAE AGPEALRWLQLYIYKDREVTKKLVR QAEKMGYKAIFVTVD TPYLGNRLDDVRNRFKLPPQLRMKN FETSTLSFSPEENFGDDSGLAAYVA KAIDPSISWEDIKWL RRLTSLPIVAKGILRGDDAREAVKH GLNGILVSNHGARQLDGVPATIDVL PEIVEAVEGKVEVFL DGGVRKGTDVLKALALGAKAVFVGR PIVWGLAFQGEKGVQDVLEILKEEF RLAMALSGCQNVKVI DKTLVRKNPLAVSKI
• Endotoxin: Less than 0.1 ng/μg (1 IEU/μg).
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Storage: Store at Please minimize freeze-thaw cycles.
• Protein length: Met1-Ile370

Gene Information

• Gene Name: HAO1 hydroxyacid oxidase (glycolate oxidase) 1 [ Homo sapiens ]
• Official Symbol: HAO1
• Synonyms: HAO1; hydroxyacid oxidase (glycolate oxidase) 1; GOX1; hydroxyacid oxidase 1; GOX; glycolate oxidase; (S)-2-hydroxy-acid oxidase; HAOX1; MGC142225; MGC142227
• Gene ID: 54363
• mRNA Refseq: NM_017545
• Protein Refseq: NP_060015
• MIM: 605023
• UniProt ID: Q9UJM8
• Chromosome Location: 20p12
• Pathway: Glyoxylate and dicarboxylate metabolism, organism-specific biosystem; Glyoxylate and dicarboxylate metabolism, conserved biosystem; Glyoxylate metabolism, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; Peroxisome, organism-specific biosystem
• Function: (S)-2-hydroxy-acid oxidase activity; FMN binding; glycolate oxidase activity; glycolate oxidase activity; glyoxylate oxidase activity; long-chain-(S)-2-hydroxy-long-chain-acid oxidase activity; medium-chain-(S)-2-hydroxy-acid oxidase activity; oxidoreductase activity; very-long-chain-(S)-2-hydroxy-acid oxidase activity

Reviews

05/09/2022

Worked well in experiments.

11/28/2020

Great for academic research.

01/28/2020

Very satisfied, will buy again.

Q&As

How is HAO1 implicated in metabolic disorders?

Dysregulation of HAO1 is linked to metabolic disorders and fatty liver disease.

Are there existing or potential therapeutic strategies targeting HAO1?

Therapeutic approaches may include modulation of HAO1 activity or expression.

How does HAO1 contribute to fatty acid metabolism?

It oxidizes specific fatty acids, contributing to their breakdown.

What are the key biological functions of HAO1?

HAO1 is involved in peroxisomal fatty acid oxidation, crucial for lipid metabolism.

What are the potential consequences of HAO1 overexpression?

HAO1 overexpression may lead to oxidative stress and liver dysfunction.

What role does HAO1 play in liver function?

HAO1 supports liver function by participating in lipid catabolism.

How is HAO1 activity regulated in cells?

Cellular regulation of HAO1 involves transcriptional and post-translational mechanisms.