Active Recombinant Human GSTA1 Protein

• Cat.No.: GSTA1-104H
• Product Overview: Recombinant human GSTA1 protein without tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Description: This gene encodes a member of a family of enzymes that function to add glutathione to target electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. This action is an important step in detoxification of these compounds. This subfamily of enzymes has a particular role in protecting cells from reactive oxygen species and the products of peroxidation. Polymorphisms in this gene influence the ability of individuals to metabolize different drugs. This gene is located in a cluster of similar genes and pseudogenes on chromosome 6. Alternative splicing results in multiple transcript variants.
• Bio-activity: GST Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 100 mM potassium phosphate buffer (pH 6.5) containing 1 mM reduced glutathione, at a protein concentration of 0.2 μg/ml, using a continuous spectrophotometric procedure)
  Substrate: 1-chloro-2,4-dinitrobenzene (CDNB)
  Vmax: 61 μmol/min/mg protein
  Km (CDNB): 0.81 mM(Km for reduced glutathione, based on average batch data, is approximately 0.15 mM)
• Usage: For laboratory (research) purposes only
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: Protein Concentration: 10 mg/ml
• Storage Buffer: 10 mM sodium phosphate (pH 7.4), 140 mM NaCl

Gene Information

• Gene Name: GSTA1 glutathione S-transferase alpha 1 [ Homo sapiens (human) ]
• Official Symbol: GSTA1
• Synonyms: GSTA1; glutathione S-transferase alpha 1; glutathione S-transferase A1; 13-hydroperoxyoctadecadienoate peroxidase; GST HA subunit 1; GST class-alpha member 1; S-(hydroxyalkyl)glutathione lyase A1; androst-5-ene-3,17-dione isomerase; glutathione S-alkyltransferase A1; glutathione S-aryltransferase A1; glutathione S-transferase 2; glutathione S-transferase Ha subunit 1; testicular tissue protein Li 80
• Gene ID: 2938
• mRNA Refseq: NM_145740
• Protein Refseq: NP_665683
• MIM: 138359
• UniProt ID: P08263