Active Recombinant Human GPRC5D protein, His-Avi&Flag-tagged, Biotinylated(Nanodisc)

• Cat.No.: GPRC5D-638H
• Product Overview: Biotinylated Recombinant Human GPRC5D protein(Q9NZD1-2)(Tyr 2 - Phe 192 & Glu 197 - Leu 262) is expressed from HEK293 with His tag, Avi tag and Flag tag.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&Flag&His
• Protein Length: Tyr 2 - Phe 192 & Glu 197 - Leu 262
• Form: Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.
• Bio-activity: Immobilized Biotinylated Human GPRC5D, His,Avitag&Flag Tag (Nanodisc) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Anti-GPRC5D Antibody, Mouse IgG1 with a linear range of 0.002-0.313 μg/mL.
• Molecular Mass: This protein carries a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus followed by an Avi tag with calculated MW of 53.5 kDa. The protein migrates as 35-40 kDa under reducing (R) condition (SDS-PAGE). The membrane scaffold protein (MSP1D1) has calculated MW of 24.7 kDa, and it migrates as 25 kDa under reducing (R) condition (SDS-PAGE).
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >85% as determined by SDS-PAGE.
• Storage: This product is stable after storage at:
  The product MUST be stored at -70°C or lower upon receipt;
  -70°C for 3 months under sterile conditions.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: GPRC5D G protein-coupled receptor, family C, group 5, member D [ Homo sapiens ]
• Official Symbol: GPRC5D
• Synonyms: GPRC5D; G protein-coupled receptor, family C, group 5, member D; G-protein coupled receptor family C group 5 member D; orphan G-protein coupled receptor; MGC129713; MGC129714
• Gene ID: 55507
• mRNA Refseq: NM_018654
• Protein Refseq: NP_061124
• MIM: 607437
• UniProt ID: Q9NZD1