Source :	HEK293
Species :	Human
Tag :	Non
Form :	Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
Bio-activity :	Elisa plates were pre-coated with Flag Tag GPR77-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with GPR77-Nanodisc is 3.415ng/ml.
Molecular Mass :	The human full length C5AR2 protein has a MW of 36.1 kDa.
Storage :	Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
Reconstitution :	It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Full Length :	Full L.

Gene Name	GPR77 G protein-coupled receptor 77 [ Homo sapiens ]
Official Symbol	GPR77
Synonyms	GPR77; G protein-coupled receptor 77; C5a anaphylatoxin chemotactic receptor C5L2; C5L2; G-protein coupled receptor 77; G protein-coupled receptor C5L2; GPF77;
Gene ID	27202
mRNA Refseq	NM_018485
Protein Refseq	NP_060955
MIM	609949
UniProt ID	Q9P296