Active Recombinant Human GNMT Protein, His-tagged, Bioactivity Validated

• Cat.No.: GNMT-232H
• Product Overview: Recombinant Human GNMT(1-295aa) fused with His tag at N-terminal was expressed in E. coli. Bioactivity Validated.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-295aa
• Description: Glycine N-methyltransferase, also known as GNMT, catalyzes the synthesis of N-methylglycine (sarcosine) from glycine using S-adenosylmethionine (AdoMet) as the methyl donor. This protein affects DNA methylation by regulating the ratio of S-adenosylmethionine to S-adenosylhomocystine and participates in the detoxification pathway in liver cells. Also it is reported that GNMT expression is diminished in human hepatocellular carcinoma (HCC).
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol
• Bio-activity: Specific activity is > 100 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C.
• Molecular Mass: 34.9 kDa (315aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MVDSVYRTRS LGVAAEGLPD QYADGEAARV WQLYIGDTRS RTAEYKAWLL GLLRQHGCQR VLDVACGTGV DSIMLVEEGF
  SVTSVDASDK MLKYALKERW NRRHEPAFDK WVIEEANWMT LDKDVPQSAE GGFDAVICLG NSFAHLPDCK GDQSEHRLAL KNIASMVRAG GLLVIDHRNY
  DHILSTGCAP PGKNIYYKSD LTKDVTTSVL IVNNKAHMVT LDYTVQVPGA GQDGSPGLSK FRLSYYPHCL ASFTELLQAA FGGKCQHSVL GDFKPYKPGQ
  TYIPCYFIHV LKRTD
• Purity: > 95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: GNMT glycine N-methyltransferase [ Homo sapiens ]
• Official Symbol: GNMT
• Synonyms: GNMT; glycine N-methyltransferase
• Gene ID: 27232
• mRNA Refseq: NM_018960
• Protein Refseq: NP_061833
• MIM: 606628
• UniProt ID: Q14749