Active Recombinant Human Glypican 6, His-tagged

• Cat.No.: GPC6-5629H
• Product Overview: RecombinantHuman GPC6 protein was expressed in Murine myeloma cell line. Asp24-Arg354& Ser355-Val527, with a C-terminal 6-His tag.

Specification

• Cat. No.: GPC6-5629H
• Description: The glypicans(glypiated proteoglycans) are a small multigene family of GPI-linkedproteoglycans that likely play a key role in embryonic morphogenesis. Thereare six known mammalian glypicans. They all share a common-sized protein coreof 60-70 kDa, an N-terminus which likely forms a compact globular domain, 14conserved cysteines that form multiple intrachain disulfide bonds, and anumber of C-terminal N-and O-linked carbohydrate attachment sites. Based onexon organization and the location of O-linked glycosylation sites, at leasttwo subfamilies of glypicans are known, with one subfamily containingglypicans-1, 2, 4, and 6, and another subfamily containing glypicans-3 and 5.
• Source: Mammalian cells
• Form: Lyophilized from a0.2 um filtered solution in PBS.
• N-terminalSequence: Asp24 & Ser355
• MolecularWeight: 38 kDa and 20 kDa
• Activity: Measuredby its binding ability in a functional ELISA. When biotinylated rmWnt-3a (0.5μg/mL, 100 μL/well) is captured on streptavidin-coated plate, rhGlypican-6binds with an apparent KD < 10 nM.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 38 kDaand 27-29 kDa, reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 100μg/mL in PBS.
• Storage: Use a manual defrost freezer and avoid repeated freezethawcycles. 12months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °Cunder sterile conditions after reconstitution. 3 months, -20 to -70 °C understerile conditions after reconstitution.
• Protein length: 24-354;355-527 a.a.

Gene Information

• Gene Name: GPC6 glypican 6 [ Homosapiens ]
• Official Symbol: GPC6
• Synonyms: GPC6; glypican 6;OMIMD1; glypican-6;glypican proteoglycan 6
• Gene ID: 10082
• mRNA Refseq: NM_005708
• Protein Refseq: NP_005699
• MIM: 604404
• UniProt ID: Q9Y625
• Chromosome Location: 13q32
• Function: heparan sulfateproteoglycan binding