Active Recombinant Human GHR Protein, Fc-tagged

• Cat.No.: GHR-1623H
• Product Overview: Recombinant Human GHR (Ala27-Tyr264) with Human IgG1 (Pro100-Lys330) Fc tag was expressed in Mouse myeloma cell line.

Specification

• Species: Human
• Source: Mouse myeloma cell
• Tag: Fc
• Protein Length: 27-264
• Description: This gene encodes a member of the type I cytokine receptor family, which is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. In humans and rabbits, but not rodents, growth hormone binding protein (GHBP) is generated by proteolytic cleavage of the extracellular ligand-binding domain from the mature growth hormone receptor protein. Multiple alternatively spliced transcript variants have been found for this gene.
• Form: Disulfide-linked homodimer
• Bio-activity: Measured by its ability to inhibit GH-induced proliferation of Nb2-11 rat lymphoma cells. The ED50 for this effect is 0.4-2.0 ng/mL in the presence of 0.2 ng/mL of rhGH.
• Molecular Mass: 54 kDa (monomer)
• N-terminal Sequence Analysis: Ala27
• Endotoxin: < 0.01 EU/μg of the protein by the LAL method.
• Purity: > 90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: GHR growth hormone receptor [ Homo sapiens (human) ]
• Official Symbol: GHR
• Synonyms: GHR; growth hormone receptor; GHBP; GHIP; growth hormone receptor; GH receptor; growth hormone binding protein; mutant growth hormone receptor; serum binding protein; somatotropin receptor; truncated growth hormone receptor
• Gene ID: 2690
• mRNA Refseq: NM_000163
• Protein Refseq: NP_000154
• MIM: 600946
• UniProt ID: P10912