Active Recombinant Human GC Protein (Leu17-Leu474, K436T), C-6×His-tagged

• Cat.No.: GC-01H
• Product Overview: Recombinant Human GC Protein (Leu17-Leu474, K436T) with a C-terminal 6-His tag was expressed in Mouse myeloma cell line.

Specification

• Description: The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: Mouse myeloma cell line
• Species: Human
• Tag: His
• Bio-activity: Measured by its binding ability in a functional ELISA. When Vitamin D3-BSA Conjugate is immobilized at 2 μg/mL, 100 μL/well, Recombinant Human Vitamin D BP binds with an ED50 of 2-10 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 52 kDa
  SDS-PAGE: 57-63 kDa, reducing conditions
• Protein length: Leu17-Leu474, K436T
• N-terminal Sequence Analysis: Leu17
• Endotoxin: <0.10 EU/μg of the protein by the LAL method.
• Purity: >90% by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: 12 months from date of receipt, ≤ -20 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: GC GC vitamin D binding protein [ Homo sapiens (human) ]
• Official Symbol: GC
• Synonyms: GC; GC vitamin D binding protein; DBP; VDB; GRD3; VDBG; VDBP; GcMAF; DBP/GC; Gc-MAF; DBP-maf; HEL-S-51; vitamin D-binding protein; epididymis secretory protein Li 51; gc protein-derived macrophage activating factor; gc-globulin; group-specific component (vitamin D binding protein); vitamin D-binding alpha-globulin; vitamin D-binding protein-macrophage activating factor; EC 6.3.1.5; EC 6.4.-.-
• Gene ID: 2638
• mRNA Refseq: NM_000583
• Protein Refseq: NP_000574
• MIM: 139200
• UniProt ID: P02774