Active Recombinant Human Ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin), His-tagged

• Cat.No.: FCN2-494H
• Product Overview: Recombinant Human FCN2is expressed in Murine myeloma cell line, which has a molecule weight of 32.8kDa. Leu26 ­ Ala313, with a C­terminal 10­His tag.

Specification

• Cat. No.: FCN2-494H
• Description: Human Ficolin­2 (fibrinogen/collagen­like;previously called L­ficolin or ficolin­B) is a member of the ficolin family ofsecreted pattern recognition proteins that participate in the lectin complementactivation pathway. Ficolin­2 is expressed in the liver and released into thecirculation. The 35 ­ 40 kDa, 313 amino acid (aa) human Ficolin­2 contains a 25aa signal sequence, an N­terminal collagen domain and a C­terminal fibrinogen­likedomain that includes a calcium binding site and two potential N­glycosylationsites. The collagen domain mediates trimer formation. Larger homo­multimers areformed by disulfide links at the N­terminus, the most prominent of which is a12 subunit oligomer. Ficolin­2 binds microbial ligands that contain acetylatedcompounds. Mature human Ficolin­2 shares 70%, 72%, 76% and 78% aa identity withmouse, rat, cow and pig Ficolin­2, respectively. It shares 84% and 52% aa identitywith human ficolin­1 and ficolin­3, respectively. Single nucleotide polymorphismsare common in human Ficolin­2. Some affect serum concentration, while others canincrease or decrease ligand binding.
• Source: Mammalian cells
• Form: Lyophilized from a 0.2μm filtered solution in PBS.
• N-terminal Sequence: Leu26
• Molecular Weight: 32.8 kDa
• Activity: Measured by its abilityto bind biotinylated N­Acetylglucosamine­Polyacrylamide in a functional ELISA.
• Purity: >95%, by SDS­PAGEunder reducing conditions and visualized by silver stain.
• Endotoxin Level: <1.0 EU per 1 μg ofthe protein by the LAL method.
• Reconstitution: Reconstitute at 100 μg/mLin PBS.
• Storage: Use a manual defrostfreezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
• OfficialSymbol: FCN2

Gene Information

• Gene Name: FCN2 ficolin (collagen/fibrinogendomain containing lectin) 2 (hucolin) [ Homo sapiens ]
• Synonyms: FCN2; ficolin (collagen/fibrinogendomain containing lectin) 2 (hucolin); P35; FCNL; EBP-37; ficolin-2; L-ficolin;ficolin B; ficolin-B; ficolin-beta; serum lectin p35; Hucolin; 37 kDa elastin-bindingprotein; collagen/fibrinogen domain-containing protein 2; OTTHUMP00000022518
• Gene ID: 2220
• mRNA Refseq: NM_004108
• Protein Refseq: NP_004099
• MIM: 601624
• UniProt ID: Q15485
• Chromosome Location: 9q34.3
• Function: antigen binding; calciumion binding; calcium-dependent protein binding; receptor binding; sugar binding