Active Recombinant Human Fibroblast Growth Factor Binding Protein 1, His-tagged

• Cat.No.: FGFBP1-548H
• Product Overview: Recombinant Human FGFBP1protein was expressed in Murine myeloma cell line with a C-terminal 10-Histag. Lys24-Cys234 & Asn29-Cys234.

Specification

• Cat. No.: FGFBP1-548H
• Description: Fibroblast growthfactor binding protein (FGFBP1), also known as HBP17, is a secretedglycoprotein that increases the bioavailability of FGFs. Mature FGFBP1 is a34 kDa, 211 amino acids (aa) O-glycosylated protein with five conserved intrachaindisulfide bonds. FGFBP1 contains a heparinbinding domain (aa 110-143) and adistinct FGF binding region (aa 193-243). Mature human FGFBP1 shares 59% and54% aa sequence identity with mouse and rat FGFBP1, respectively. FGFBP1 isexpressed throughout development and in adult squamous epithelium.
• Source: Mammalian cells
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Lys24 & Asn29
• Molecular Weight: 25.2 kDa& 24.6 kDa
• Activity: Measuredby its binding ability in a functional ELISA. 1 μg/mL of rhFGFBP1 was mixedwith serially diluted rhFGF basic. Following incubation, the FGFBP-FGF basiccomplex was captured on a Gt x hFGFBP-coated plate. Bound FGF basic wasmeasured using biotinylated Gt x hFGF basic. The concentration of rhFGF basicthat produces 50% of the optimal binding was found to be approximately 5-20ng/mL.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 31-35kDa, reducing conditions
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 100 μg/mL in sterile PBS
• Storage: Use a manualdefrost freezer and avoid repeated freeze-thaw cycles. 12 months from date ofreceipt, -20 to -70°C as supplied. 1 month, 2 to 8 °C under sterile conditionsafter reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution.
• Protein length: 24-234;29-234 a.a.

Gene Information

• Gene Name: FGFBP1 fibroblast growth factorbinding protein 1 [ Homo sapiens ]
• Official Symbol: FGFBP1
• Synonyms: FGFBP1; fibroblastgrowth factor binding protein 1; FGFBP; HBP17; FGF-BP; FGF-BP1; FGFBP-1;FGF-binding protein 1; 17 kDa heparin-binding growth factor-binding protein;17 kDa HBGF-binding protein; heparin-binding growth factor binding protein
• Gene ID: 9982
• mRNA Refseq: NM_005130
• Protein Refseq: NP_005121
• MIM: 607737
• UniProt ID: Q14512
• Chromosome Location: 4p15.32
• Function: fibroblast growthfactor binding; heparin binding; protein binding