Active Recombinant Human FGF4 (54 - 206aa)

• Cat.No.: FGF4-023E
• Product Overview: Recombinant Human FGF4 (Ser54 ­ Leu206) was expressed in E. coli and produced using non­animal reagent in an animal­free laboratory.

Specification

• Cat. No.: FGF4-023E
• Description: FGF­4, the product of a developmentally regulated gene (hst­1), is a member of the FGF family that is efficiently secreted. The gene for FGF­4 (also known as FGFK of K­FGF for Kaposi sarcoma­associated FGF) was initially discovered as a transforming gene by the NIH ­3T3 focus formation assay using DNA derived from human tumors (including stomach and colon cancers, hepatocellular carcinomas, and Kaposi"s sarcomas). FGF­4 does not seem to be expressed in normal adult tissues. However, expression of the gene is spatially and temporally regulated during embryonic development. In vitro, FGF­4 is mitogenic for fibroblasts and endothelial cells. FGF­4 has been shown to be a potent angiogenesis promoter in vivo. FGF­4 has potent transforming potential apparently through an autocrine mechanism of action. FGF­4 plays a key role in limb development and has been identified as the molecular mediator of the activities of the apical ectodermal ridge that is required for directing the outgrowth and patterning of vertebrate limbs.
• Source: E. coli
• N­terminal Sequence Analysis: Ser54, Leu55
• Molecular Mass: 17 kDa
• SDS­PAGE: 16 kDa, reducing conditions
• Activity: Measured in a cell proliferation assay using NR6R­3T3 mouse fibroblasts. The ED50 for this effect is typically 0.25 ­ 1.25 ng/mL.
• Endotoxin Leve: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >97%, by SDS­PAGE under reducing conditions and visualized by silver stain.
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS. See Certificate of Analysis for details.
• Reconstitution: Reconstitute at 0.2 mg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
• Storage: Use a manual defrost freezer and avoid repeated freeze­thaw cycles. 1. 12 months from date of receipt, ­20 to ­70 °C as supplied. 2. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3. 3 months, ­20 to ­70 °C under sterile conditions after reconstitution.
• Unitprot ID: P08620
• Tag: Non

Gene Information

• Gene Name: FGF4 fibroblast growth factor 4 [ Homo sapiens ]
• Official Symbol: FGF4
• Synonyms: FGF4; fibroblast growth factor 4; HST; KFGF; HST-1; HSTF1; K-FGF; HBGF-4; FGF-4; HSTF-1; oncogene HST; kaposi sarcoma oncogene; transforming protein KS3; heparin-binding growth factor 4; heparin secretory transforming protein 1; heparin secretory-transforming protein 1; fibroblast growth factor 4 splice isoform; human stomach cancer, transforming factor from FGF-related oncogene
• Gene ID: 2249
• mRNA Refseq: NM_002007
• Protein Refseq: NP_001998
• MIM: 164980
• Chromosome Location: 11q13.3
• Pathway: Downstream signaling of activated FGFR; FGF signaling pathway; FGFR ligand binding and activation; FRS2-mediated cascade; IRS-mediated signaling; Insulin receptor signalling cascade; MAPK signaling pathway; PI3K Cascade; Regulation of actin cytoskeleton
• Function: fibroblast growth factor receptor binding; growth factor activity; heparin binding; protein tyrosine kinase activity