Active Recombinant Human FCGR2A protein, His-tagged

Cat.No. : FCGR2A-399H
Product Overview : Recombinant Human FCGR2A protein(P12318-1)(Met1-Ile218,167H), fused with His tag, was expressed in CHO.
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Source : CHO
Species : Human
Tag : His
Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : Measured by its binding ability in a functional ELISA.Immobilized human CD32a-His (R167H) (CHO) at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.09-0.21 μg/ml.
Molecular Mass : The secreted recombinant human CD32a consists of 196 amino acids and predicts a molecular mass of 22 KDa. The apparent molecular mass of the protein is approximately 29-32 KDa in SDS-PAGE under reducing conditions due to glycosylation.
Protein length : Met1-Ile218,167H
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 95 % as determined by SDS-PAGE
Storage : Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name FCGR2A Fc fragment of IgG, low affinity IIa, receptor (CD32) [ Homo sapiens ]
Official Symbol FCGR2A
Synonyms FCGR2A; Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc fragment of IgG, low affinity IIa, receptor for (CD32) , FCG2, FCGR2, FCGR2A1; low affinity immunoglobulin gamma Fc region receptor II-a; CD32; CD32A; CDw32; IGFR2; Immunoglobulin G Fc receptor II; fcRII-a; fc-gamma-RIIa; fc-gamma RII-a; igG Fc receptor II-a; FCG2; FcGR; FCGR2; FCGR2A1; MGC23887; MGC30032;
Gene ID 2212
mRNA Refseq NM_001136219
Protein Refseq NP_001129691
MIM 146790
UniProt ID P12318

Not For Human Consumption!

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