Active Recombinant Human FCGR2A Protein, His-tagged

• Cat.No.: FCGR2A-06H
• Product Overview: Recombinant Human Fc gamma RIIA/CD32a (H167) Protein (Gln34-Ile218), with a C-terminal 10-His tag, was expressed in Mouse myeloma cell line.

Specification

• Species: Human
• Source: Mouse myeloma cell
• Tag: His
• Protein Length: 34-218 a.a.
• Description: This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.
• Bio-activity: Measured by its binding ability in a functional ELISA. When Recombinant Human Fc gamma RIIA/CD32a (H167) is coated at 1 μg/mL, 100 μL/well, it binds to human IgG with an ED50 of 0.25‑1.25 μg/mL.
• Molecular Mass: 22 kDa
• N-terminal Sequence Analysis: Ala36 identified. Gln34 inferred from enzymatic pyroglutamate treatment revealing Ala35
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 95% by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: FCGR2A Fc gamma receptor IIa [ Homo sapiens (human) ]
• Official Symbol: FCGR2A
• Synonyms: FCGR2A; Fc gamma receptor IIa; CD32; FCG2; FcGR; CD32A; CDw32; FCGR2; IGFR2; FCGR2A1; FcgammaRIIa; low affinity immunoglobulin gamma Fc region receptor II-a; Fc fragment of IgG receptor IIa; Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc gamma receptor RIIa3; Immunoglobulin G Fc receptor II; fc-gamma-RIIa; fcRII-a; igG Fc receptor II-a
• Gene ID: 2212
• mRNA Refseq: NM_021642
• Protein Refseq: NP_067674
• MIM: 146790
• UniProt ID: P12318