Active Recombinant Human FBP1 Protein, His-tagged

• Cat.No.: FBP1-389H
• Product Overview: Recombinant FBP1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Fructose-1,6-bisphosphatase 1(FBP1) is a gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Fructose-1,6-diphosphatase deficiency is associated with hypoglycemia and metabolic acidosis.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol.
• Bio-activity: Specific activity is >7,000 pmol/min/ug obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from thereduction of NADP. One unit will oxidize 1.0 pmole of fructose 1,6 diphosphate to fructose 6-phosphate and inorganic phosphate
• Molecular Mass: 39.0 kDa (358aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MADQAPFDTD VNTLTRFVME EGRKARGTGE LTQLLNSLCT AVKAISSAVR KAGIAHLYGI AGSTNVTGDQ VKKLDVLSND LVMNMLKSSF ATCVLVSEED KHAIIVEPEK RGKYVVCFDP LDGSSNIDCL VSVGTIFGIY RKKSTDEPSE KDALQPGRNLVAAGYALYGS ATMLVLAMDC GVNCFMLDPA IGEFILVDKD VKIKKKGKIY SLNEGYARDF DPAVTEYIQR KKFPPDNSAP YGARYVGSMV ADVHRTLVYG GIFLYPANKK SPNGKLRLLY ECNPMAYVME KAGGMATTGK EAVLDVIPTD IHQRAPVILG SPDDVLEFLK VYEKHSAQ
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: FBP1 fructose-1,6-bisphosphatase 1 [ Homo sapiens ]
• Official Symbol: FBP1
• Synonyms: FBP1; fructose-1,6-bisphosphatase 1; FBP; FBPase 1; fructose-bisphosphatase 1; growth-inhibiting protein 17; D-fructose-1,6-bisphosphate 1-phosphohydrolase 1
• Gene ID: 2203
• mRNA Refseq: NM_000507
• Protein Refseq: NP_000498
• MIM: 611570
• UniProt ID: P09467
• Chromosome Location: 9q22.3
• Pathway: Fructose and mannose metabolism, organism-specific biosystem; Fructose and mannose metabolism, conserved biosystem; Gluconeogenesis, organism-specific biosystem; Gluconeogenesis, oxaloacetate =>fructose-6P, organism-specific biosystem; Gluconeogenesis, oxaloacetate => fructose-6P, conserved biosystem
• Function: AMP binding; fructose 1,6-bisphosphate 1-phosphatase activity; fructose 1,6-bisphosphate 1-phosphatase activity; fructose 1,6-bisphosphate 1-phosphatase activity; hydrolase activity; metal ion binding; monosaccharide binding; monosaccharide binding; phosphoric ester hydrolase activity; protein binding