Active Recombinant Human F9 protein, His-tagged

• Cat.No.: F9-1157H
• Product Overview: Recombinant Human F9 protein(AAB59620.1)(Met1-Thr461), fused with His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Thr461
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave the peptide substrate, Z-D-Arg-Gly-Arg-pNA. The specific activity is >20pmols/min/ug.
• Molecular Mass: The secreted recombinant human F9 (pro form) consists of 444 amino acids and predictes a molecular mass of 50 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh F9 is approximately 60-80 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: F9 coagulation factor IX [ Homo sapiens ]
• Official Symbol: F9
• Synonyms: F9; coagulation factor IX; Christmas disease; Factor IX; FIX; hemophilia B; plasma thromboplastic component; F9 p22; FIX F9; factor 9; factor IX F9; serine protease; Christmas factor; plasma thromboplastin component; P19; PTC; HEMB; THPH8; MGC129641; MGC129642
• Gene ID: 2158
• mRNA Refseq: NM_000133
• Protein Refseq: NP_000124
• MIM: 300746
• UniProt ID: P00740