Active Recombinant Human EPHB6, Fc tagged

• Cat.No.: EPHB6-40H
• Product Overview: Recombinant Human EPHB6 precursor extracellular domain (Met 1-Ser 579) (NP_004436.1), fused with the Fc region of human IgG1 at the C-terminus, was produced in Human Cell.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: Met 1-Ser 579
• Form: Lyophilized from sterile PBS, pH 7.4
• Bio-activity: 1. Measured by its binding ability in a functional ELISA.
  2. Immobilized recombinant human EphrinB1 at 10 μg/ml (100 μl/well) can bind human EphB6 with a linear range of 0.16-4 μg/ml.
  3. Immobilized recombinant human EphrinB2 at 10 μg/ml (100 μl/well) can bind human EphB6 with a linear range of 1.28-32 ng/ml.
• Molecular Mass: The recombinant mature human EphB6/Fc chimera is a disulfide linked homodimeric protein. Each monomer consists of 801 amino acids and has a calculated molecular mass of 86.5 kDa. In SDS-PAGE under reducing conditions, rhEphB6/Fc monomer migrates with an apparent molecular mass of approximately 100-110 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EPHB6 EPH receptor B6 [ Homo sapiens ]
• Official Symbol: EPHB6
• Synonyms: EPHB6; EPH receptor B6; EphB6; ephrin type-B receptor 6; HEP; tyrosine-protein kinase-defective receptor EPH-6; MGC129910; MGC129911
• Gene ID: 2051
• mRNA Refseq: NM_004445
• Protein Refseq: NP_004436
• MIM: 602757
• UniProt ID: O15197