Active Recombinant Human EPHB2 protein(Gly570-Val987), His&GST-tagged

• Cat.No.: EPHB2-7299H
• Product Overview: Recombinant Human EPHB2 (P29323-3) (Gly570-Val987) was expressed in Insect Cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: Gly570-Val987
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 3mM DTT, 10% gly.
• Bio-activity: The specific activity was determined to be 120 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substRate.
• Molecular Mass: The recombinant human EPHB2 /GST chimera consists of 655 amino acids and has a calculated molecular mass of 75.2 kDa. The recombinant protein migRates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EPHB2 EPH receptor B2 [ Homo sapiens ]
• Official Symbol: EPHB2
• Synonyms: EPHB2; EPH receptor B2; DRT, EphB2 , EPHT3, ERK; ephrin type-B receptor 2; Hek5; Tyro5; EPH-like kinase 5; eph tyrosine kinase 3; elk-related tyrosine kinase; protein-tyrosine kinase HEK5; tyrosine-protein kinase TYRO5; renal carcinoma antigen NY-REN-47; tyrosine-protein kinase receptor EPH-3; developmentally-regulated Eph-related tyrosine kinase; DRT; EK5; ERK; CAPB; PCBC; EPHT3; MGC87492
• Gene ID: 2048
• mRNA Refseq: NM_004442
• Protein Refseq: NP_004433
• MIM: 600997
• UniProt ID: P29323