Active Recombinant Human ENTPD3, His-tagged

• Cat.No.: ENTPD3-208H
• Product Overview: A DNA sequence encoding the human ENTPD3 extracellular domain (Gln 44-Pro 485) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.

Specification

• Description: This gene encodes a plasma membrane-bound divalent cation-dependent E-type nucleotidase. The encoded protein is involved in the regulation of extracellular levels of ATP by hydrolysis of it and other nucleotides. Multiple transcript variants have been described.
• Source: Baculovirus-Insect Cells
• Species: Human
• Tag: His
• Predicted N Terminal: Gln 44
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly
• Bio-activity: Measured by its ability to hydrolyze the 5’-phosphate groups from the substrate adenosine-5’-triphosphate (ATP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit.The specific activity is >70,000 pmol/min/µg.
• Molecular Mass: The recombinant human ENTPD3 (aa44-485) consists of 452 amino acids and predicts a molecular mass of 51 kDa. It migrates as an approximately 50 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70?
• Storage: Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
• Protein length: Gln44-Pro485

Gene Information

• Gene Name: ENTPD3 ectonucleoside triphosphate diphosphohydrolase 3 [ Homo sapiens (human) ]
• Official Symbol: ENTPD3
• Synonyms: ENTPD3; ectonucleoside triphosphate diphosphohydrolase 3; CD39L3; HB6; NTPDase 3; CD39-like 3; ecto-ATPase 3; ecto-ATPDase 3; ecto-apyrase 3; CD39 antigen-like 3; ecto-ATP diphosphohydrolase 3; NTPDase-3; FLJ93839
• Gene ID: 956
• mRNA Refseq: NM_001248
• Protein Refseq: NP_001239
• MIM: 603161
• UniProt ID: O75355
• Chromosome Location: 3p21.3
• Pathway: Epstein-Barr virus infection; Purine metabolism; Pyrimidine metabolism
• Function: ATP binding; nucleoside-diphosphatase activity