Active Recombinant Human ENTPD2 Protein, His tagged

• Cat.No.: ENTPD2-01H
• Product Overview: Active Recombinant Human ENTPD2 Protein (Thr29-Asp460) with a C-terminal 6-His tag was expressed in Chinese Hamster Ovary cell line.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Protein Length: 29-460 aa
• Description: The protein encoded by this gene is the type 2 enzyme of the ecto-nucleoside triphosphate diphosphohydrolase family (E-NTPDase). E-NTPDases are a family of ecto-nucleosidases that hydrolyze 5''-triphosphates. This ecto-ATPase is an integral membrane protein. Alternative splicing of this gene results in multiple transcript variants.
• Form: Monomer
• Molecular Mass: 48 kDa
• N-terminal Sequence Analysis: Thr29
• Bio-Activity: Measured by its ability to hydrolyze the 5''-phosphate groups from the substrate adenosine-5''-triphosphate (ATP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit. The specific activity is > 5,000 pmol/min/μg, as measured under the described conditions.
• Endotoxin: < 1 EU/μg by LAL.
• Purity: > 95 % by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl2 and Glycerol.
• Shipping: The product is shipped with polar packs
• Reference: 1. Zimmermann, H. et al. 2000 Proceedings of the Second International Workshop on EctoATPases and Related Ectonucleotidases:18.
  2. Robson, S.C. et al. 2001 Drug Dev. Res. 53:193.
  3. Marcus, A.J. et al. 2005 Semin. Thromb. Hemost. 31:234.

Gene Information

• Gene Name: ENTPD2 ectonucleoside triphosphate diphosphohydrolase 2 [ Homo sapiens (human) ]
• Official Symbol: ENTPD2
• Synonyms: ENTPD2; ectonucleoside triphosphate diphosphohydrolase 2; CD39L1; CD39 like 1; ecto ATPase; NTPDase 2; ecto-ATPase 2; ecto-ATPDase 2; CD39 antigen-like 1; ecto-ATP diphosphohydrolase 2; NTPDase-2
• Gene ID: 954
• mRNA Refseq: NM_001246
• Protein Refseq: NP_001237
• MIM: 602012
• UniProt ID: Q9Y5L3