Active Recombinant Human EIF2AK2 Protein, GST-tagged

• Cat.No.: EIF2AK2-3148H
• Product Overview: Human EIF2AK2 (NP_002750.1, 252 a.a. - 551 a.a.) partial recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Protein Length: 252-551 a.a.
• Description: The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
• Form: Liquid
• Bio-activity: The activity was determined by IMAPTM assay. The enzyme was incubated with fluorescein labeled peptide and phosphorylation was detected by IMAPTM technology (fluorescence polarization).
  Substrate: SRPKtide.
  ATP: 100 μM.
• Molecular Mass: 62 kDa
• Purity: 61 % by SDS-PAGE/CBB staining
• Applications: Functional Study
  SDS-PAGE
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)

Gene Information

• Gene Name: EIF2AK2 eukaryotic translation initiation factor 2-alpha kinase 2 [ Homo sapiens ]
• Official Symbol: EIF2AK2
• Synonyms: EIF2AK2; eukaryotic translation initiation factor 2-alpha kinase 2; PRKR, protein kinase, interferon inducible double stranded RNA dependent; interferon-induced, double-stranded RNA-activated protein kinase; EIF2AK1; PKR; p68 kinase; eIF-2A protein kinase 2; P1/eIF-2A protein kinase; tyrosine-protein kinase EIF2AK2; interferon-inducible elF2alpha kinase; double stranded RNA activated protein kinase; protein kinase, interferon-inducible double stranded RNA dependent; PRKR; MGC126524
• Gene ID: 5610
• mRNA Refseq: NM_001135651
• Protein Refseq: NP_001129123
• MIM: 176871
• UniProt ID: P19525