Active Recombinant Human EGFL7 protein(Tyr24-Ser273), His-tagged

• Cat.No.: EGFL7-3077H
• Product Overview: Recombinant Human EGFL7 (NP_057299.1) (Tyr 24-Ser 273) was expressed in Insect Cells, fused with a polyhistidine tag at the N-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: His
• Protein length: Tyr24-Ser273
• Form: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 10% glycerol, pH 7.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When 5 × 10E4 cells/well areadded to EGFL7-His coated plates (1.25μg/mL and 100μL/well),approximately >45% will adhere specifically after 30 minutes at 37°C.
• Molecular Mass: The recombinant human EGFL7 consists of 268 amino acids and predicts a molecular mass of 29.4 kDa. It migRates as an approximately 31 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EGFL7 EGF-like-domain, multiple 7 [ Homo sapiens ]
• Official Symbol: EGFL7
• Synonyms: EGFL7; EGF-like-domain, multiple 7; epidermal growth factor-like protein 7; ZNEU1; EGF-like protein 7; NOTCH4-like protein; vascular endothelial statin; multiple EGF-like domains protein 7; multiple epidermal growth factor-like domains protein 7; NEU1; VE-STATIN; RP11-251M1.2; MGC111117
• Gene ID: 51162
• mRNA Refseq: NM_016215
• Protein Refseq: NP_057299
• MIM: 608582
• UniProt ID: Q9UHF1